Team II Functional Annotation Group

Introduction

Data

We are given 258 assembled genomes and predicted genes of Klebsiella spp.

Non-coding RNA

rRNA, tRNA, and sRNA

Please refer to "Team II Gene Prediction Group - RNA Prediction" [1]

CRISPR

CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are found in approximately 40% of sequenced bacterial genomes which are found to be related to Bacterial immunity regulation, cell defense mechanism, DNA rearrangement, replication, and regulation. And Klebsiella has an unusually high proportion of self-targeting spacers

Tool

Version: Piler-CR 1.06
PILER-CR is a program which specifically designed for the identification and analysis of CRISPR repeats. The program executes rapidly and has both high sensitivity and high specificity.

Input: FASTA

Output: Piler-CR specific report format

Performance by using reference genome:

Run-time: ~5 seconds for a 5Mb genome
CRISPR found:2