Team I Webserver Group
Web Server
Introduction
Background
The goal of Klebsiella Antibiotics REsistance PredicitioN (KAREN) webserver is to assemble and annotate genome of Klebsiella spp. and provide the results to the user in an user-friendly format. KAREN could also be used to assemble genomes of other bacteria, however the server has been currently designed to annotate only Klebsiella genomes.
The objective of the BIOL7210: Computational Genomics this year was to perform genome assembly and functionally annotate 258 genomes. Recent studies have shown the emergence of colistin and fosfomycin resistance within Klebsiella spp..
KAREN is able to perform the following analyses with the input of raw sequence reads.
- TO DO: - TO DO: - TO DO:
Goals
- Assemble input reads
- Analyze assemblies
- Visualize results
- Implement a way for results to be downloaded
Technologies Used
For the creation and development of this webserver
Functionalities
De Novo Genome Assembly
FastQC was used to perform quality control checks on the raw input sequence data. Then, de novo sequencing was used in our pipeline because no reference sequence is needed in this case. Sequence reads are assembled as contigs, and the coverage quality of de novo sequence data depends on the size and continuity of the contigs. We used Skesa for de novo genome assembly. This tool is currently unpublished.
Species & Strain Typing
MEGA, GenomeTester4 and StrainSeeker were used to constructs a list of specific k-mers for each node of any given Newick-format tree and enables the identification of bacterial isolates in 1–2 min. MEGA7 was used to align the sequences and construct neighbor-joining tree. Then StrainSeeker was used to build a custom database using the 258 Klebsiella genomes we were given. To build a custom database, the tree generated by MEGA7 was used to function as the guide tree, describing the relationships between given strains. Then StrainSeeker was used to detect novel strains that are related to strains in the database.
perl builder.pl -n refseq_guide_tree.nwk -d strain_fasta_directory -w 32 -o my_database
perl seeker.pl -i sample_file.fastq -d ss_db_w32 -o sample_result.txt
A pre-build database is used by the StrainSeeker for species identification. Strainseeker is a tool which lets you rapidly and accurately makes as assessment of the species and strain of a bacterial assembly. It works in a matter of minutes and can be customized to use a user-created database. It works on paired-end reads and can even identify novel strains and place them near their close relatives on the phylogeny tree. It is therefore a useful tool for further assessment of a sample of unknown origin.
For KAREN, we are specifically concerned only with Klebsiella spp.. When testing the results using the pre-built database, our results showed it was seemed accurate at analyzing the Klebsiella strains. For this reason, we choose to use the pre-built database for finding species and strain identification.
Genome Database
We created a curated list of genes that are included in the 258 Klebsiella genomes we were given, performed a literature review to find genes that may indicate colistin resistance, and built a gene panel to help us find phenotypic indicators in our assembled genomes. We will be using a MySQL database that will show 0 (absent) or 1 (present) for both antibiotic resistance genes and virulence factor genes.